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Digital immunoassay · interactive laboratory
How does
Simoa work?
An interactive resource to explore protein capture, microwell readout and the relationship between counting, concentration and reaction volume.
Open interactive laboratory →What you will explore
- Sandwich immunoassay steps on beads.
- Positive-well readout and Poisson correction.
- The relationship between enzymatic product confinement and local concentration.
- Calibration, nonspecific background and sample matrix.
Before you start
This resource is hosted by CTMM and uses illustrative simulations. Values are not kit specifications, CTMM experimental results or clinical decision criteria. Consult the protocol and validation of the assay used in each study.
Source and context
Detection in femtoliter arrays is described by Rissin et al., Nature Biotechnology, 2010. Consult Infrastructure for the institutional platform status.