Learn / Droplet digital PCR
Molecular biology · interactive explanation
Droplet digital PCR
Learn how reaction partitioning and Poisson correction estimate target copies. Explore droplet occupancy, sampling uncertainty and rare-variant detection.
Open interactive laboratory →Follow
From reaction preparation to endpoint fluorescence reading.
Experiment
Change concentration and droplet count; observe Poisson correction and uncertainty.
Interpret
Compare DNA input, mutant fraction, false-positive background and analyzed volume.
Educational model with synthetic data. Droplet volume and false positives are illustrative assumptions. Sampling probability is not clinical sensitivity; this resource does not validate assays or describe CTMM equipment or available services.